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Immunology and Infection

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JoVE Journal Immunology and Infection
Verzadigde vetzuren induceren Ceramide-geassocieerde Macrophage celdood
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Verzadigde vetzuren induceren Ceramide-geassocieerde Macrophage celdood

Article DOI: 10.3791/56535-v • 08:26 min • October 31st, 2017
October 31st, 2017 •
1, 1, 1, 1
1Department of Microbiology and Immunology, University of Louisville

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We illustreren een straight-forward methode om te ontlenen lymfkliertest primaire macrofagen van beenmergcellen en een eenvoudige methode om te bereiden BSA-vetzuur conjugaten. Dan tonen we dat verzadigde vetzuren leiden de dood van de cel van de macrofaag tot kan, en dergelijke celdood positief geassocieerd met cellulaire accumulatie van ceramide niveaus wordt.

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Immunologie kwestie 128 beenmerg-afgeleide macrofagen BSA (bovien serumalbumine) verzadigd vetzuren palmitinezuur stearinezuur ceramide celdood
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