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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Mettede fettsyrer indusere celledød Ceramide-assosiert Macrophage
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Mettede fettsyrer indusere celledød Ceramide-assosiert Macrophage

Article DOI: 10.3791/56535
October 31st, 2017 •
1, 1, 1, 1
1Department of Microbiology and Immunology, University of Louisville

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October 31st, 2017

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Vi illustrerer en rett fram metode til å utlede murine primære makrofager fra benmargen celler og en enkel metode å forberede BSA-fettsyrer conjugates. Deretter viser vi at mettede fettsyrer kan indusere macrophage celledød, og slike celledød er positivt assosiert med mobilnettet akkumulering av ceramide nivåer.

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