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Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down
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Cancer Research
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JoVE Journal Cancer Research
Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

DOI:
10.3791/56583-v

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08:59 min

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December 11, 2017

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, , , , , ,
1Biosciences Laboratory,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Chapters

  • 00:05Title
  • 01:39Generation of Targeted Therapy Resistant Subclones
  • 04:03Restoring Target-therapy Inhibitor Sensitivity by Candidate R-gene Knock-down
  • 06:31Results: Drug Resistant Subclone Generation and Sensitivity Restoration
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

This is a time- and cost-effective in vitro protocol investigating the mechanisms of acquired resistance to targeted therapeutic agents, which is a highly unmet medical need in cancer management.

Tags

Keywords: – Acquired Resistance – Targeted Therapy – Drug Resistant Subclone – Sensitivity Restoration – Gene Knock-down – Tumor Heterogeneity – Mechanism Of Resistance – Preclinical Platform – Trastuzumab – HER-positive Gastric Cancer – In-vitro Drug Resistant Model – Gradually Increasing Drug Dose – Plasma Peak Concentration

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