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Immunology and Infection
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Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
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Immunology and Infection
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JoVE Journal Immunology and Infection
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

DOI:
10.3791/56605-v

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07:50 min

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December 08, 2017

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, , ,
1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Chapters

  • 00:05Title
  • 00:35Nickel Affinity Chromatography
  • 03:30Heparin Affinity Chromatography
  • 04:28Nuclease Assay
  • 06:46Results: Nuclease Assay of Heparin Sepharose Fractions
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

Recombinant prototype foamy virus integrase protein is often contaminated with a bacterial nuclease during purification. This method identifies nuclease contamination and removes it from the final preparation of the enzyme.

Tags

Keywords: Nuclease ContaminationProtein PurificationRecombinant Prototype Foamy Virus IntegraseE. ColiSonicationUltracentrifugationFPLCNickel-charged ResinSDS-PAGEUV Absorbance

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