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Journal / Bioengineering / Fabrikation af brugerdefinerede Agarosen Wells…
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Bioengineering

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JoVE Journal Bioengineering
Fabrikation af brugerdefinerede Agarosen Wells for celle såning og væv Ring samlesæt bruger 3D-trykt forme
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Fabrikation af brugerdefinerede Agarosen Wells for celle såning og væv Ring samlesæt bruger 3D-trykt forme

Article DOI: 10.3791/56618-v • 08:16 min • April 2nd, 2018
April 2nd, 2018 •
1, 1, 1, 1,2, 1
1Biomedical Engineering, Worcester Polytechnic Institute, 2Gladstone Institute for Cardiovascular Disease

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Denne protokol beskriver en platform for at fabrikere selvsamlede væv ringe i variabel størrelser ved hjælp af et tilpassede 3D-trykt plast skimmel. PDMS negativer er helbredt i formen 3D-trykt; Agarosen er støbt i de hærdede PDMS negativer. Cellerne udsås i de resulterende Agarosen brønd hvor de samlede i væv ringe.

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Bioteknologi spørgsmål 134 3D udskrivning selvsamlede væv karvæv engineering agarosegelelektroforese brugerdefinerede skimmel vævsmanipulering
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