Low-field (L-band, 1.2 GHz) electron paramagnetic resonance using soluble nitroxyl and trityl probes is demonstrated for assessment of physiologically important parameters in the tumor microenvironment in mouse models of breast cancer.
Bobko, A. A., Eubank, T. D., Driesschaert, B., Khramtsov, V. V. In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment. J. Vis. Exp. (133), e56624, doi:10.3791/56624 (2018).