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Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)
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1Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 2Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 3The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services,Rutgers Robert Wood Johnson Medical School, 4The Child Health Institute of NJ, Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 5Department of Genetics,Rutgers University
Chapters
- 00:04Title
- 01:08Collecting NPCs for Plating of Experimental Conditions
- 02:30Neural Precursor Cell DNA Synthesis Assay
- 03:47S Phase Entry Assay
- 04:39Neural Precursor Cell Number Assay
- 05:41Neural Precursor Cell Neurosphere Migration Assay
- 07:58Results: Use of Phase-contrast and Fluorescence Microscopy to Study the Proliferation, Migration, and Neurite Outgrowth of Neural Precursor Cells
- 09:51Conclusion
Neurodevelopmental processes such as proliferation, migration, and neurite outgrowth are often perturbed in neuropsychiatric diseases. Thus, we present protocols to rapidly and reproducibly assess these neurodevelopmental processes in human iPSC-derived NPCs. These protocols also allow the assessment of the effects of relevant growth factors and therapeutics on NPC development.
