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Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse
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Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse

Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse

DOI:
10.3791/56648-v

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03:52 min

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March 02, 2018

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,
1National Institute on Deafness and Other Communication Disorders,National Institutes of Health, 2Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins School of Medicine

Chapters

  • 00:05Title
  • 00:32Procedure Setup
  • 01:08Surgical Approach
  • 02:35Results: Transduction of Utricular and Cochlear Hair Cells
  • 03:22Conclusion

Summary

Automatic Translation

Automatic Translation

In this study, we describe the posterior semicircular canal approach as a reliable method for inner ear gene delivery in neonatal mice. We show that gene delivery through the posterior semicircular canal is able to perfuse the entire inner ear.

Tags

Keywords: Posterior Semicircular CanalInner Ear Gene DeliveryNeonatal MouseAAV8-whirlinGene TherapyHair Cell TransductionStereocilia ElongationSurgical DissectionMicro-injection

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