Journal
/
Medicine
/
Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

DOI:
10.3791/56672-v

•

08:13 min

•

January 07, 2018

•

,
1Department of Laboratory Medicine,Medical University of Vienna

Chapters

  • 00:05Title
  • 00:42Oral Glucose Tolerance Test
  • 04:10Insulin Tolerance test
  • 05:45Results: Glucose Resistance and Compensatory Hyperinsulinemia in Mice Fed a HFD
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

The current article describes the generation and metabolic characterization of high-fat diet-fed mice as a model of diet-induced insulin resistance and obesity. It further features detailed protocols to perform the oral glucose tolerance test and the insulin tolerance test, monitoring whole-body alterations of glucose metabolism in vivo.

Tags

Keywords: In Vivo Glucose MetabolismHigh-fat DietMiceOral Glucose Tolerance Test (OGTT)Insulin Tolerance Test (ITT)Metabolic PhenotypeGlucose MetabolismGenetic FactorsPharmacological FactorsDietary FactorsBody WeightBlood GlucoseGlucose SolutionGlucose AdministrationGavage

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56672?article_type=v

Making Sense of Listening: The IMAP Test Battery
check_url/56672?article_type=v

The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva
check_url/56672?article_type=v

Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice
check_url/56672?article_type=v

An in vivo Assay to Test Blood Vessel Permeability
check_url/56672?article_type=v

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test
check_url/56672?article_type=v

Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test
check_url/56672?article_type=v

Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT
check_url/56672?article_type=v

Improving Strength, Power, Muscle Aerobic Capacity, and Glucose Tolerance through Short-term Progressive Strength Training Among Elderly People
check_url/56672?article_type=v

Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain
check_url/56672?article_type=v

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Read Article