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Genetics

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Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
 

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Article DOI: 10.3791/56693
February 22nd, 2018

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Summary February 22nd, 2018

This protocol describes a heat shock-induced protein expression system (pDHsp/V5-His/sf9 cell system), which can be used for either expressing foreign proteins or evaluating the anti-apoptotic activity of potential foreign proteins and their truncated amino acids in insect cells.

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