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Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment
 

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Article doi: 10.3791/56712
January 13th, 2018

Summary January 13th, 2018

We describe an in vitro assay to model chorioallantoic attachment, the first step in placenta formation. The protocol demonstrates the dissection and explant culture of murine allantoides on immobilized α4β1 integrin. Allantois attachment is evaluated microscopically at pre-determined time points.

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