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Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment
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Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

DOI:
10.3791/56712-v

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09:30 min

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January 13, 2018

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, ,
1Institute of Pharmacology and Toxicology,University of Würzburg, 2Rudolf Virchow Center for Experimental Biomedicine,University of Würzburg

Chapters

  • 00:05Title
  • 00:42Coating of Microtiter Plates with α4β1 Integrin for Ex Utero Allantois Explant Culture
  • 01:54Uterus Dissection from a Pregnent Mouse at DPC 8
  • 03:24Embryo and Allantois Dissection and Ex Utero Allantois Cultre
  • 06:18Staining Explanted Allantoides with Endothelial Cell Marker CD31
  • 08:07Results: Allantois Attachment and Immunohistochemistry
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

We describe an in vitro assay to model chorioallantoic attachment, the first step in placenta formation. The protocol demonstrates the dissection and explant culture of murine allantoides on immobilized α4β1 integrin. Allantois attachment is evaluated microscopically at pre-determined time points.

Tags

Keywords: Murine AllantoisAllantoic AttachmentPlacenta FormationEmbryo DevelopmentIn Vitro AnalysisAlpha 4/beta 1 IntegrinExplant CultureDissectionUterusMouse Embryo

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