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Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
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Cancer Research
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JoVE Journal Cancer Research
Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

DOI:
10.3791/56739-v

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12:34 min

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April 02, 2018

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, ,
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital,Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital,Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,Central South University, 4The State Key Laboratory of Medical Genetics,Central South University

Chapters

  • 00:04Title
  • 01:46Two-dimensional Gel Electrophoresis: IEF (First Dimension)
  • 03:12Two-dimensional Gel Electrophoresis: SDS-PAGE (Second Dimension)
  • 07:19Staining of 2DE-separated Proteins
  • 08:52Results: Separation and Identification of NFPA Tissue Proteome
  • 10:33Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a two-dimensional gel electrophoresis (2DE) coupled with mass spectrometry (MS) to separate and identify human pituitary adenoma tissue proteome, which presents a good and reproducible 2DE pattern. Many proteins are observed in each 2DE spot when analyzing complex cancer proteome with the use of high-sensitivity MS.

Tags

Keywords: Two-dimensional Gel ElectrophoresisMass SpectrometryPituitary AdenomaProteome AnalysisProtein SeparationProtein IdentificationLow Abundance ProteinsIsoelectric FocusingPAGE GelProtein Sample Preparation

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