Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Xianquan Zhan; Yuda Huang; Ying Long

doi:10.3791/56739

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Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

13,467 Views

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12:34 min

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April 02, 2018

DOI:

10.3791/56739-v

DOI:

10.3791/56739-v

•

12:34 min

April 02, 2018

•

13460 Views

Xianquan Zhan^1,2,3,4, Yuda Huang^1,2,3, Ying Long^1,2,3

¹Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital,Central South University ²Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital,Central South University ³State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,Central South University ⁴The State Key Laboratory of Medical Genetics,Central South University

Summary

Here, we present a two-dimensional gel electrophoresis (2DE) coupled with mass spectrometry (MS) to separate and identify human pituitary adenoma tissue proteome, which presents a good and reproducible 2DE pattern. Many proteins are observed in each 2DE spot when analyzing complex cancer proteome with the use of high-sensitivity MS.