/
/
To-dimensionelle gelelektroforese kombineret med massespektrometri metoder til analyse af menneskelige hypofyseadenom væv proteom
A subscription to JoVE is required to view this content. Sign in or start your free trial.
13,166 Views
•
12:34 min
•
April 02, 2018
DOI:
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital,Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital,Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,Central South University, 4The State Key Laboratory of Medical Genetics,Central South University
Summary
Automatically generated
Her præsenterer vi en to-dimensionel gelelektroforese (2DE) kombineret med massespektrometri (MS) til at adskille og identificere menneskelige hypofyseadenom væv proteomet, som præsenterer en god og reproducerbare 2DE mønster. Mange proteiner er observeret i hver 2DE plet når analysere komplekse kræft proteomet med brug af høj-følsomhed MS.
Read Article
Cite this Article
Zhan, X., Huang, Y., Long, Y. Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome. J. Vis. Exp. (134), e56739, doi:10.3791/56739 (2018).
Copy