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January 02, 2018
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This manuscript describes how incision-like lesions made on cultured epithelial cell monolayers conveniently model wound healing in vitro, allowing for imaging by confocal or laser scanning microscopy, and which can provide high-quality quantitative and qualitative data for studying both cell behavior and the mechanisms involved in migration.
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Cite this Article
Liarte, S., Bernabé-García, Á., Armero-Barranco, D., Nicolás, F. J. Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing. J. Vis. Exp. (131), e56799, doi:10.3791/56799 (2018).
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