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Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing
Microscopy Based Methods for the Assessment of Epithelial Cell Migration During  <em>In Vitro</em> Wound Healing
JoVE Journal
Medicine
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JoVE Journal Medicine
Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

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08:34 min

January 02, 2018

DOI:

08:34 min
January 02, 2018

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Summary

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This manuscript describes how incision-like lesions made on cultured epithelial cell monolayers conveniently model wound healing in vitro, allowing for imaging by confocal or laser scanning microscopy, and which can provide high-quality quantitative and qualitative data for studying both cell behavior and the mechanisms involved in migration.

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