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JoVE Journal
Developmental Biology

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Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos
 

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

Article DOI: 10.3791/56836 10:28 min November 30th, 2017
November 30th, 2017

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Summary

Here, we present a simple method to quantitate hematopoietic stem and progenitor cells (HSPCs) in embryonic zebrafish. HSPCs from dissociated zebrafish are plated in methylcellulose with supportive factors, differentiating into mature blood. This allows the detection of blood defects and allows drug screening to be easily conducted.

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Tags

In Vitro Assay Hematopoietic Stem Cells Progenitor Cells Zebrafish Embryos Quantitation Differentiation Hematopoietic Field Quick Inexpensive Easy To Perform Drug Effect Genetic Mutation Blood Progenitor Formation Knockdown Genes Normally Developing Embryos Morphants Bleach Zebrafish Embryos Wash Stations
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