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Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos
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Developmental Biology
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JoVE Journal Developmental Biology
Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

DOI:
10.3791/56836-v

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10:28 min

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November 30, 2017

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,
1Department of Biological Sciences,California State University, Chico

Chapters

  • 00:05Title
  • 01:05Bleach and Dechorionate 48 hpf Zebrafish Embryos
  • 03:19Embryos for Dissociation
  • 05:15Preparation of Dissociated Embryos
  • 06:21Preparation of Methylcellulose
  • 07:30Addition of Dissociated Embryos to Methylcellulose
  • 08:53Results: Quantitation of Zebrafish Embryonic Hematopoietic Stem and Progenitor Cells
  • 09:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a simple method to quantitate hematopoietic stem and progenitor cells (HSPCs) in embryonic zebrafish. HSPCs from dissociated zebrafish are plated in methylcellulose with supportive factors, differentiating into mature blood. This allows the detection of blood defects and allows drug screening to be easily conducted.

Tags

Keywords: In Vitro AssayHematopoietic Stem CellsHematopoietic Progenitor CellsZebrafish EmbryosBlood Progenitor FormationGene KnockdownMorphantsBleachingDechorionationE3 Medium

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