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Biology

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JoVE Journal Biology
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
 

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Article doi: 10.3791/56845
March 19th, 2018 • Usage Statistics
1, 1, 1, 1, 1, 1, 2, 1,3, 1,4
1Sheba Medical Center, 2Children's Hospital of Pittsburgh of UPMC, 3Ariel University, 4Cincinnati Children's Hospital Medical Center

Summary
Automatic Translation
March 19th, 2018

This manuscript describes a detailed standardized protocol of high-throughput 16S rRNA-amplicon sequencing. The protocol introduces an integrated, uniformed, feasible, and inexpensive protocol starting from fecal sample collection through data analyses. This protocol enables analysis of large numbers of samples with rigorous standards and several controls.

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