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JoVE Journal
Biochemistry

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A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells
 

A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

Article DOI: 10.3791/56855
August 22nd, 2018

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Summary August 22nd, 2018

A standard Western blotting protocol was optimized for analyzing as few as 500 hematopoietic stem or progenitor cells. Optimization involves careful handling of the cell sample, limiting transfers between tubes, and directly lysing the cells in Laemmli sample buffer.

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