Journal
/
Developmental Biology
/
Generation and Culturing of Primary Human Keratinocytes from Adult Skin
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

DOI:
10.3791/56863-v

•

10:42 min

•

December 22, 2017

•

1Department of Dermatology,Aarhus University Hospital

Chapters

  • 00:05Title
  • 00:52Isolation of Keratinocytes from Human Skin
  • 05:32Passaging of Keratinocytes
  • 07:32Freezing Keratinocytes
  • 08:48Results: Primary Human Keratinocyte Culture from Adult Skin
  • 10:06Conclusion

Summary

Automatic Translation

Automatic Translation

The human skin acts as a first line of defense against the external environment. We present a method for isolating primary human keratinocytes from adult skin. These isolated keratinocytes are useful in numerous experimental setups, and are a highly suitable model for studying molecular mechanisms in cutaneous biology in vitro.

Tags

Keywords: Primary Human KeratinocytesAdult SkinEpidermal Cell BiologySkin DiseasesTrypsinDPBSKSFMRPMI 1640FBSGentamicinEpidermal CellsDermal Compartment

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56863?article_type=v

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
check_url/56863?article_type=v

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
check_url/56863?article_type=v

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
check_url/56863?article_type=v

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
check_url/56863?article_type=v

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
check_url/56863?article_type=v

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
check_url/56863?article_type=v

The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens
check_url/56863?article_type=v

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
check_url/56863?article_type=v

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
check_url/56863?article_type=v

Generation of Multicellular Human Primary Endometrial Organoids

Read Article