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Immunology and Infection

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Fabrication de Surfaces de verre de qualité optique pour étudier la Fusion des macrophages

Article DOI: 10.3791/56866-v • 08:50 min • March 14th, 2018
March 14th, 2018 •
1,2, 3,4,5, 6, 7, 7, 8, 1,2, 3,4,5, 1,2
1Center for Metabolic and Vascular Biology, Mayo Clinic, 2Molecular and Cellular Biosciences, School of Life Sciences, Arizona State University, 3Department of Physics, Arizona State University, 4Center for Biological Physics, Arizona State University, 5Biodesign Institute, Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 7Advanced Imaging Center, HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility, European Molecular Biology Laboratory

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Ce protocole décrit la fabrication de surfaces de verre de qualité optique adsorbé avec des composés contenant des hydrocarbures à chaîne longue qui peuvent être utilisés pour surveiller la fusion des macrophages de spécimens vivants et permet de microscopie de Super-résolution d’échantillons fixés .

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Rétraction numéro 133 formation de cellules géantes fusion multinucléaires de Macrophage imagerie live réaction à corps étranger cellules géantes de corps étranger inflammation protocole
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