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JoVE Journal
Bioengineering

This content is Open Access.

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating
 

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating

Article DOI: 10.3791/56879 05:35 min June 23rd, 2018
June 23rd, 2018

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Summary

This protocol aims to achieve surface engineering of pancreatic islets using a heparin-incorporated starPEG nanocoating via pseudo-bioorthogonal chemistry between the N-hydroxysuccinimide groups of the nanocoating and the amine groups of islet cell membrane.

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Tags

Surface Engineering Pancreatic Islets Heparinized StarPEG Nanocoating Transplantation Challenges Immuno-rejection Post-transplantation Revascularization Graft Function Therapeutic Efficacy Cell-based Therapies Low Cell Retention Poor Survival Jingyi Yang Heparin NHS EDC Solution Ethanol StarPEG MI Aid
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