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Journal / Genetics / Metaboliske mærkning og profilering af overførsel …
JoVE Journal
Genetics

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JoVE Journal Genetics
Metaboliske mærkning og profilering af overførsel RNA'er ved hjælp af Macroarrays
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Metaboliske mærkning og profilering af overførsel RNA'er ved hjælp af Macroarrays

Article DOI: 10.3791/56898-v • 10:56 min • January 16th, 2018
January 16th, 2018 •
*1, *1, *1, 1, 2, 2, 3, 1
1Laboratory of tRNA Biology, Department of Biology, College of Charleston, 2Department of Biochemistry and Molecular Biology, MUSC, 3Department of Biology, College of Charleston
* These authors contributed equally

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Vi beskriver en oprindelige transfer RNA analyse platform kaldet SPOt (strømlinet Platform for observere tRNA). SPOt samtidig foranstaltninger cellulære niveauer af alle tRNAer i biologiske prøver, i kun tre trin, og i mindre end 24 timer.

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Genetik spørgsmålet 131 overførsel ribonucleic syrer radioaktive orthofosfat macroarrays metaboliske mærkning Gen-ekspression genetiske oversættelse proteinsyntese ikke-kodende ribonucleic syrer
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