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Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
DOI:

08:51 min

April 03, 2018

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Chapters

  • 00:05Title
  • 00:45Preparation for Reverse Transcription
  • 01:57Reverse Transfection
  • 04:32Infection
  • 05:52Fixing and Staining Cells
  • 06:39Results: Genome-wide RNAi Screen to Identify Host Targets that Enhance Oncolytic Virus Therapy
  • 08:13Conclusion

Summary

Automatic Translation

Here we describe a protocol for employing high-throughput RNAi screening to uncover host targets that can be manipulated to enhance oncolytic virus therapy, specifically rhabodvirus and vaccinia virus therapy, but it can be readily adapted to other oncolytic virus platforms or for discovering host genes that modulate virus replication generally.

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