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Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
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1Children’s Hospital of Eastern Ontario (CHEO) Research Institute, 2Department of Biology, Microbiology and Immunology,University of Ottawa, 3Department of Pediatrics,University of Ottawa, 4Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine,University of Calgary
Chapters
- 00:05Title
- 00:45Preparation for Reverse Transcription
- 01:57Reverse Transfection
- 04:32Infection
- 05:52Fixing and Staining Cells
- 06:39Results: Genome-wide RNAi Screen to Identify Host Targets that Enhance Oncolytic Virus Therapy
- 08:13Conclusion
Here we describe a protocol for employing high-throughput RNAi screening to uncover host targets that can be manipulated to enhance oncolytic virus therapy, specifically rhabodvirus and vaccinia virus therapy, but it can be readily adapted to other oncolytic virus platforms or for discovering host genes that modulate virus replication generally.
