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JoVE Journal
Genetics

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A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
 

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells

Article DOI: 10.3791/56915-v 10:42 min December 12th, 2017
December 12th, 2017

Chapters

Summary

We describe the production strategy of integrase-deficient lentiviral vectors (IDLVs) as vehicles for delivering CRISPR/Cas9 to cells. With an ability to mediate quick and robust gene editing in cells, IDLVs present a safer and equally effective vector platform for gene delivery compared to integrase-competent vectors.

Tags

Keywords: Integrase-deficient Lentiviral Vectors CRISPR/Cas9 Gene Editing HEK-293T Cells Transfection Sucrose Gradient Ultracentrifugation
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