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JoVE Journal Developmental Biology
Celle aggregering analyser evaluere bindingen Drosophila hakket med Trans-ligander og dens hemming av Cis-ligander
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Celle aggregering analyser evaluere bindingen Drosophila hakket med Trans-ligander og dens hemming av Cis-ligander

Article DOI: 10.3791/56919-v • 05:49 min • January 2nd, 2018
January 2nd, 2018 •
1, 1,2
1Department of Molecular and Human Genetics, Baylor College of Medicine, 2Program in Developmental Biology, Baylor College of Medicine

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Kompleksiteten i vivo systemer gjør det vanskelig å skille mellom aktivering og hemming hakk reseptoren av trans- og cis-ligander, henholdsvis. Her presenterer vi en protokoll basert på i vitro celle-aggregering analyser for kvalitativ og kvantitativ semi evaluering av binding av Drosophila hakk til trans-ligander vs cis-ligander.

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Utviklingspsykologi biologi problemet 131 Drosophila S2 cellene cellen aggregering trans-binding cis-hemming hakket
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