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JoVE Journal Developmental Biology
Celle sammenlægning Assays til at evaluere bindingen af Drosophila Notch med Trans-ligander og dens hæmning af Cis-ligander
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Celle sammenlægning Assays til at evaluere bindingen af Drosophila Notch med Trans-ligander og dens hæmning af Cis-ligander

Article DOI: 10.3791/56919-v • 05:49 min • January 2nd, 2018
January 2nd, 2018 •
1, 1,2
1Department of Molecular and Human Genetics, Baylor College of Medicine, 2Program in Developmental Biology, Baylor College of Medicine

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I vivo systemers kompleksitet gør det vanskeligt at skelne mellem aktivering og hæmning af Notch receptor af trans- og cis-ligander, henholdsvis. Vi præsenterer her, en protokol baseret på in vitro- celle-sammenlægning assays til kvalitative og semi-kvantitative evaluering af bindingen af Drosophila hak til trans-ligander vs cis-ligander.

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Udviklingsmæssige biologi sag 131 Drosophila S2 celler celle sammenlægning trans-bindende cis-hæmning hakket
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