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JoVE Journal Neuroscience
Neuron-Macrophage co culturen te activeren van macrofagen afscheidende moleculaire factoren met Neurite uitgroei activiteit
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Neuron-Macrophage co culturen te activeren van macrofagen afscheidende moleculaire factoren met Neurite uitgroei activiteit

Article DOI: 10.3791/56920-v • 08:52 min • March 30th, 2018
March 30th, 2018 •
1,2, 1,2, 1,2,3
1Department of Brain Science, Ajou University School of Medicine, 2Neuroscience Graduate Program, Department of Biomedical Sciences, Ajou University School of Medicine, 3Department of Neurology, Ajou University School of Medicine

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Het huidige protocol presenteert experimentele procedures ter stimulering van gekweekte macrofagen te worden begiftigd met capaciteit om moleculaire factoren ter bevordering van de uitgroei van de neurite vrij te geven. Behandeling van kamp tot de neuron-macrofaag co culturen induceert de macrofagen produceren geconditioneerde medium dat sterke neurite uitgroei activiteit bezit.

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Neurowetenschappen kwestie 133 Macrophage dorsal root ganglia neuron co cultuur axon regeneratie neuron-macrofaag interactie cyclisch ADENOSINEMONOFOSFAAT conditionering letsel
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