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Journal / Cancer Research / Characterization of Tumor Cells Using a Medical…
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Cancer Research

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JoVE Journal Cancer Research
Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells
Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
Journal (Cancer Research)
Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
Extraction of Extracellular Vesicles from Whole Tissue
Journal (Cancer Research)
Extraction of Extracellular Vesicles from Whole Tissue
 

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

Article DOI: 10.3791/56936-v • 10:56 min • December 21st, 2017
December 21st, 2017 •
1, 2, 3, 1, 1
1Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Associazione Annastaccatolisa Onlus, 3Nuclear Medicine Unit, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

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Summary
Automatic Translation

We present a new approach to characterize tumor cells. We combined immunofluorescence with DNA fluorescent-in-situ-hybridization to evaluate cells captured by a functionalized medical wire capable of in vivo enriching CTCs directly from patient blood.

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Tumor Cells Medical Wire Circulating Tumor Cells Immunofluorescence DNA FISH 3D Approach Immunophenotype FISH Signals Widefield Fluorescence Microscope Non-small-cell Lung Cancer Cells EpCAM Positivity Cytosomatic Alterations ARC Gene Status Putative CTCs In Situ Insulin Cancer CTC Features Breast Cancer Immunophenotyping Protocol DNA Fluorescence In Situ Hybridization Protocol Handling The Wire Functionalized Tip
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