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Kombinere røntgenkrystallografi med lille vinkel X-Ray spredning til Model ustruktureret regioner af Nsa1 fra S. Cerevisiae
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Chapters
- 00:05Title
- 00:47Nsa1 (Nop7 Associated 1) Protein Purification
- 03:43Proteolytic Screening
- 05:14Small-Angle X-Ray Scattering (SAXS) Data Processing, Comparison of Concentration Series, and Ensemble Modeling
- 07:31Results: Modeling the Disordered C-Terminus Region of Nsa1
- 08:37Conclusion
Denne metode beskriver kloning, udtryk og oprensning af rekombinante Nsa1 for Strukturbestemmelse af røntgenkrystallografi og små-vinkel X-ray spredning (SAXSA), og er gældende for hybrid strukturel analyse af andre proteiner der indeholder begge bestilt og uordnede domæner.
Tags
X-ray CrystallographySmall Angle X-ray Scattering (SAXS)Structural AnalysisProtein StructureRecombinant ProteinUnstructured RegionsNsa1S. CerevisiaeHybrid Structural AnalysisProtein FunctionProtein DynamicsProtein AggregationPurificationAffinity ChromatographyGel FiltrationSDS-PAGETEV ProteaseCentrifugal Filtration
