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Kombinere røntgenkrystallografi med lille vinkel X-Ray spredning til Model ustruktureret regioner af Nsa1 fra S. Cerevisiae
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
DOI:

09:15 min

January 10, 2018

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Chapters

  • 00:05Title
  • 00:47Nsa1 (Nop7 Associated 1) Protein Purification
  • 03:43Proteolytic Screening
  • 05:14Small-Angle X-Ray Scattering (SAXS) Data Processing, Comparison of Concentration Series, and Ensemble Modeling
  • 07:31Results: Modeling the Disordered C-Terminus Region of Nsa1
  • 08:37Conclusion

Summary

Automatic Translation

Denne metode beskriver kloning, udtryk og oprensning af rekombinante Nsa1 for Strukturbestemmelse af røntgenkrystallografi og små-vinkel X-ray spredning (SAXSA), og er gældende for hybrid strukturel analyse af andre proteiner der indeholder begge bestilt og uordnede domæner.

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