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JoVE Journal Biochemistry
Het combineren van röntgendiffractie met kleine hoek X-Ray Scattering model ongestructureerde regio's van de Nsa1 van S. Cerevisiae
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Het combineren van röntgendiffractie met kleine hoek X-Ray Scattering model ongestructureerde regio's van de Nsa1 van S. Cerevisiae

Article DOI: 10.3791/56953-v • 09:15 min • January 10th, 2018
January 10th, 2018 •
1, 1, 1
1Signal Transduction Laboratory, National Institutes of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health

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Deze methode beschrijft de klonen, expressie en zuivering van recombinante Nsa1 voor structurele bepaling door middel van röntgendiffractie en small-angle X-ray scattering (SAXS), en is van toepassing voor de hybride structurele analyse van andere eiwitten die beide bevatten besteld en ontregelde domeinen.

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Biochemie kwestie 131 röntgendiffractie SAXS hybride methoden EiwitReiniging WD40 domein beperkte proteolyse de assemblage van het ribosoom
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