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Journal / Bioengineering / Large-area Scanning Probe Nanolithography…
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Bioengineering

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JoVE Journal Bioengineering
Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies
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Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Article DOI: 10.3791/56967-v • 09:45 min • June 12th, 2018
June 12th, 2018 •
*1,2, *1, 3, 4, 2, 3, 1
1Manchester Institute of Biotechnology & School of Chemistry, University of Manchester, 2School of Engineering, University of Liverpool, 3School of Electrical and Electronic Engineering, University of Manchester, 4School of Science and Technology, Nottingham Trent University
* These authors contributed equally

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Summary
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Here we present a protocol for wide-area scanning probe nanolithography enabled by the iterative alignment of probe arrays, as well as the utilization of lithographic patterns for cell-surface interaction studies.

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Large-area Scanning Probe Nanolithography Automated Alignment Substrate Fabrication Cell Culture Studies Nanometer-scale Features Nanostructured Materials Protein Arrays Mesenchymal Stem Cells PDMS Prepolymer Vacuum Desiccator Silicon Master PPL Array Glass Slide Oxygen Plasma
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