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In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
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In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

DOI:
10.3791/57032-v

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10:22 min

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October 19, 2018

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, , , , , , , ,
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center,Soochow University, 2Department of Oncology,The First Affiliated Hospital of Soochow University

Chapters

  • 00:04Title
  • 01:18Plating and Maintenance of Caco-2bbe on Porous Polycarbonate Membranes
  • 02:53Use of Epithelial Meter for Measuring Transepithelial Electrical Resistance
  • 04:39Murine Model of Dextran Sulfate Sodium (DSS)-induced Colitis
  • 06:07Measuring the Epithelial Barrier Permeability in DSS-induced Colitis Mice
  • 08:01Results: Measure of Permeability of Intestinal Epithelial Cells
  • 09:29Conclusion

Summary

Automatic Translation

Automatic Translation

Two methods are presented here to determine intestinal barrier function. An epithelial meter (volt/ohm) is used for measurements of transepithelial electrical resistance of cultured epithelia directly in tissue culture wells. In mice, the FITC-dextran gavage method is used to determine the intestinal permeability in vivo.

Tags

Keywords: Intestinal Epithelial Cell PermeabilityIn VitroIn VivoInflammatory Bowel DiseaseGastrointestinal DiseasesBarrier IntegrityCaco-2bbe CellsTransepithelial Electrical Resistance (TER)Interferon Gamma

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