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Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

DOI:
10.3791/57036-v

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09:52 min

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April 26, 2018

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, , , ,
1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology,Kindai University, 2Faculty of Biology-Oriented Science and Technology,Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology,University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture,Kyoto University, 5Institute of Advanced Technology,Kindai University

Chapters

  • 00:05Title
  • 00:52Preparation of Deionized Bovine Serum Albumin
  • 01:48Oocyte Collection
  • 03:26Preparation of Donor Cells for Nuclear Transfer and Enucleation of Oocytes
  • 05:32Fusion of Donor Cell and Enucleated Oocyte
  • 07:42Activation of Reconstructed Oocytes and Treatment with Trichostatin A and Vitamin C
  • 08:26Results: Somatic Cell Nuclear Transfer in Mice using Trichostatin A and Vitamin C Treatment
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a dramatically improved method for mouse cloning using trichostatin A, vitamin C, and deionized bovine serum albumin. We show a simplified, reproducible protocol that supports efficient development of cloned embryos. Hence, this method could become a standardized procedure for mouse cloning.

Tags

Keywords: Somatic Cell Nuclear TransferCloningMouseEmbryonic DevelopmentNuclear ProgrammingGenetic Research ConservationBSAIon Exchange ResinSodium BicarbonateEuthanasiaSuperovulationOviductsCumulus Oocyte ComplexesHyaluronidaseHCZB MediumMKSOM MediumDBSAEnucleation

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