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Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation
 

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Article doi: 10.3791/57056
May 3rd, 2018

Summary May 3rd, 2018

This method can be used to measure RNA synthesis. 5-Bromouridine is added to cells and incorporated into synthesized RNA. RNA synthesis is measured by RNA extraction immediately after labelling, followed by 5-Bromouridine-targeted immunoprecipitation of labelled RNA and analysis by reverse transcription and quantitative polymerase chain reaction.

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