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Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

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11:19 min

March 20, 2018

DOI:

11:19 min
March 20, 2018

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Summary

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This protocol targets specific cells in tissue for imaging at nanoscale resolution using a scanning electron microscope (SEM). Large numbers of serial sections from resin-embedded biological material are first imaged in a light microscope to identify the target and then in a hierarchical manner in the SEM.

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