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JoVE Journal
Developmental Biology

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Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
 

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Article doi: 10.3791/57059
March 20th, 2018

Summary March 20th, 2018

This protocol targets specific cells in tissue for imaging at nanoscale resolution using a scanning electron microscope (SEM). Large numbers of serial sections from resin-embedded biological material are first imaged in a light microscope to identify the target and then in a hierarchical manner in the SEM.

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