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Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR
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Cancer Research
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JoVE Journal Cancer Research
Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

DOI:
10.3791/57066-v

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09:16 min

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February 05, 2018

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, , , , ,
1Biosciences Laboratory,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Department of Biology and Biotechnology,University of Pavia

Chapters

  • 00:04Title
  • 01:03Preparing the PCR Reaction Mixes and the PCR Chip
  • 04:31Digital PCR (dPCR)
  • 05:30Chip Analysis
  • 07:05Results: Screening for CDH1a Expression in Tumor Tissue
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

The manuscript describes a chip-based digital PCR assay to detect a rare CDH1 transcript variant (CDH1a) in fresh-frozen normal and tumor tissues obtained from patients with gastric cancer.

Tags

Keywords: CDH1Rare Transcript VariantDigital PCRChip-basedLow-abundant TargetsCDNAQuantitative PCRBiological SamplesMaster MixAssayNo Template ControlPrimerCentrifugationChip LoaderImmersion FluidLoading Blade

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