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Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development
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Developmental Biology
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JoVE Journal Developmental Biology
Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

DOI:
10.3791/57068-v

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10:30 min

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June 12, 2018

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, , ,
1Faculty of Life and Environmental Sciences, Department of Biotechnology,University of Yamanashi, 2Department of Integrated Bioscience, Graduate School of Frontier Sciences,University of Tokyo, 3Advanced Biotechnology Center,University of Yamanashi

Chapters

  • 00:05Title
  • 01:01Preparation of Chamber and Microinjection Pipettes and Microinjection
  • 03:42zFRAP Analysis
  • 08:22Results: zFRAP Analysis of Chromatin Looseness for Full Term Development of Mice
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

Chromatin looseness appears to be involved in the developmental potential of blastomeres. However, it is not known whether chromatin looseness can be used as a reliable index for the developmental potential for embryos. Here, an experimental system in which chromatin looseness-evaluated zygotes can develop to full term has been described.

Tags

Keywords: Zygotic FRAPZFRAPChromatin LoosenessEmbryonic DevelopmentEGFP-H2BMRNA InjectionMicroinjectionConfocal MicroscopyPhoto-bleaching

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