Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / Optimizing the Genetic Incorporation of Chemical…
JoVE Journal
Chemistry

A subscription to JoVE is required to view this content.

JoVE Journal Chemistry
Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
Journal (Chemistry)
Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
Microfluidic Preparation of Liquid Crystalline Elastomer Actuators
Journal (Chemistry)
Microfluidic Preparation of Liquid Crystalline Elastomer Actuators
 

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Article DOI: 10.3791/57069-v • 14:02 min • April 9th, 2018
April 9th, 2018 •
*1, *1, *1, 1
1Institute of Biochemistry, Faculty of Life Sciences, University of Leipzig
* These authors contributed equally

Chapters

Summary
Automatic Translation

A facile fluorescence assay is presented to evaluate the efficiency of amino-acyl-tRNA-synthetase/tRNA pairs incorporating non-canonical amino-acids (ncAAs) into proteins expressed in mammalian cells. The application of ncAAs to study G-protein coupled receptors (GPCRs) is described, including photo-crosslinking mapping of binding sites and bioorthogonal GPCR labeling on live cells.

Tags

Genetic Incorporation Chemical Probes GPCRs Photo-crosslinking Mapping Bioorthogonal Chemistry Live Mammalian Cells Non-canonical Amino Acids Protein-protein Interaction Surfaces Amber Codon Suppression Fluorescence Assay Ligand Binding Sites Intake Receptor Bioorthogonal Labeling Site-specific Labeling Catalyst-free Labeling Small Fluorophores High-end Imaging Applications
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter