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Development of Recombinant Proteins to Treat Chronic Pain
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Development of Recombinant Proteins to Treat Chronic Pain

Development of Recombinant Proteins to Treat Chronic Pain

DOI:
10.3791/57071-v

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10:37 min

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April 11, 2018

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, , , , ,
1Laboratory of Translational Immunology,University Medical Center Utrecht, Utrecht University, 2Department of Rheumatology & Clinical Immunology,University Medical Center Utrecht, Utrecht University, 3Laboratory of Neuroimmunology and Developmental Origins of Disease,University Medical Center Utrecht, Utrecht University

Chapters

  • 00:05Title
  • 01:15Cell Transfection, Protein Purification, and Affinity Chromatrography
  • 03:49Bioactivity of IL4-10 Fusion Protein
  • 04:46Induction of Chronic Inflammatory Pain in Mice. Pain Measurements: The Von Frey Test: Mechanical Sensitivity
  • 05:52Pain measurements: Hargreaves Test for Thermal Sensitivity Dynamic Weight Bearing Test for Postural Deficits
  • 06:48Intrathecal Injection of IL4-10 Fusion Protein for Analgesia
  • 07:56Results: Persistent Inflammatory Pain Reduction Using IL4-10 Fusion Protein
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

In this paper we provide details for the methods of production and quality control of an IL4-10 recombinant fusion protein. We also show how to test the effectiveness of this protein to resolve pain in a mouse model of inflammatory pain.

Tags

Recombinant ProteinsIL4-10 Fusion ProteinChronic PainAnti-inflammatory CytokinesProtein ProductionProtein PurificationHEK 293F CellsTransfectionAffinity ChromatographyDialysisSDS-PAGE

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