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Immunology and Infection

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JoVE Journal Immunology and Infection
Extração de hemócitos de larvas de Drosophila melanogaster para infecção microbiana e análise
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Extração de hemócitos de larvas de Drosophila melanogaster para infecção microbiana e análise

Article DOI: 10.3791/57077-v • 08:54 min • May 24th, 2018
May 24th, 2018 •
1, 2, 1
1School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, 2Integrative Physiology and Neuroscience, College of Veterinary Medicine, Washington State University

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Este método demonstra como visualizar a invasão do patógeno em células de inseto com modelos tridimensionais (3D). Hemócitos de larvas de Drosophila foram infectados com patógenos virais ou bacterianos, ou ex vivo ou no vivo. Hemócitos infectados foram, em seguida, fixa e manchados para a imagem latente com um microscópio confocal e subsequente reconstrução celular 3D.

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Imunologia e infecção edição 135 invasão do patógeno extração hematócito microscopia confocal Coxiella burnetii Listeria monocytogenes invertebrados iridescente vírus-6 IIV6
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