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A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
JoVE Journal
Medicine
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JoVE Journal Medicine
A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

DOI:
10.3791/57079-v

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10:35 min

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April 05, 2018

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, , ,
1Biodesix

Chapters

  • 00:04Title
  • 01:13Reverse Transcription of RNA to cDNA
  • 02:23Digital PCR
  • 05:29Data Analysis and Review and Generation of Results
  • 07:27Results
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

Detection of circulating ribonucleic acid (cRNA) from blood is an unmet need in clinical diagnostics. Here we describe methods that characterize cRNA from non-small cell lung cancer patients using sensitive and specific digital polymerase chain reaction. The tests meet design requirements to detect fusion variants within 72 hours.

Tags

Keywords: Blood-based TestROS1RETFusion TranscriptsCirculating Ribonucleic AcidDigital Polymerase Chain ReactionTumor-derived RNAOncology DiagnosticsMinimally InvasiveNSCLCReverse TranscriptionCDNAPCRFluorescent Probes

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