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Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
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Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Article DOI: 10.3791/57081-v • 08:12 min • March 29th, 2018
March 29th, 2018 •
1,2, 1,2, 1,2, 1,2, 1,2
1Institut National de la Santé et Recherche Médicale (INSERM) UMRS 1138, Paris-Diderot University, Pierre & Marie Curie University, Paris-Descartes University, Laboratory of Molecular Oral Pathophysiology, Cordeliers Research Centre, 2Unit of Formation and Research (UFR) of Odontology, Paris-Diderot University

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Summary
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Understanding enamel formation and possible alterations requires the study of ameloblast activity. Here, we describe a reliable and consistent method to micro-dissect enamel organs containing secretion- and maturation-stage ameloblasts that may be used for further quantitative and qualitative experimental procedures.

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Micro-dissection Enamel Organ Rat Mandibular Incisor Environmental Toxicants Dental Epithelium Cell Differentiation Enamel Synthesis Enamel Pathologies Dental Fluorosis Molar Incisor Hyperenamelization Hereditary Enamel Genesis Imperfectum Quantitative Experiments Immunohistochemistry Endogenous Molecules Exogenous Molecules Disrupt Hemogenesis Euthanizing Lower Lips Skin Removal Lower Incisors Incision Mandible Splitting Temporal-mandibular Joint Cutting Jaw Detachment Soft Tissues Removal Muscles Removal Tendons Removal Ligaments Removal
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