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1Institut National de la Santé et Recherche Médicale (INSERM) UMRS 1138, Paris-Diderot University, Pierre & Marie Curie University, Paris-Descartes University, Laboratory of Molecular Oral Pathophysiology,Cordeliers Research Centre, 2Unit of Formation and Research (UFR) of Odontology,Paris-Diderot University
Chapters
- 00:04Title
- 01:07Dissection of Hemi-mandibles from Adult Rats and Isolation of Incisor
- 03:14Micro-dissection of Enamel Organs
- 04:42Collection of Separated Enamel Organ Tissues for Further Analyses
- 06:02Results: Characterizing Ameloblasts
- 07:28Conclusion
Understanding enamel formation and possible alterations requires the study of ameloblast activity. Here, we describe a reliable and consistent method to micro-dissect enamel organs containing secretion- and maturation-stage ameloblasts that may be used for further quantitative and qualitative experimental procedures.
Tags
Keywords:
Enamel OrganMandibular IncisorRatsEnvironmental ToxicantsDental EpitheliumEnamel SynthesisEnamel PathologiesDental FluorosisMolar Incisor HyperenamelizationHereditary Enamel Genesis ImperfectumImmunohistochemistryHemogenesisEuthanasiaHemi-mandibleTemporal-mandibular JointCervical LoopAmeloblastsPBS
