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JoVE Journal
Biochemistry

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Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
 

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Article DOI: 10.3791/57092
December 12th, 2017

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Summary December 12th, 2017

A procedure is presented for the refolding of the dCACHE periplasmic ligand binding domain of Campylobacter jejuni chemoreceptor Tlp3 from inclusion bodies and the purification to yield milligram quantities of protein.

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