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Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies

Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies

DOI:
10.3791/57100-v

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12:32 min

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March 13, 2018

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, , ,
1Department of Microbiology and Immunology,Geisel School of Medicine at Dartmouth

Chapters

  • 00:04Title
  • 01:07Enzymatic Digestion of Tissues
  • 02:34Physical Separation of Single Cells
  • 04:57Dead Cell Removal
  • 06:00DC Purification by Positive Magnetic Bead Selection
  • 08:02Allogeneic Stimulation Assay to Assess DC Cell Function
  • 10:19Results: Successful Isolation of DCs from the Human Female Reproductive Tract
  • 11:55Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here a method to isolate and purify dendritic cells from different anatomical compartments in the human female reproductive tract for the evaluation of their phenotypical and functional characteristics. This method can be adapted to isolate other immune cells or dendritic cells from other mucosal tissues.

Tags

Keywords: Dendritic CellsFemale Reproductive TractPhenotypicalFunctionalImmune CellsEpithelial SheetsEnzymatic DigestionCell Isolation

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