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Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
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Chapters
- 00:04Title
- 00:45Cultivation of Animals
- 03:08Lipid Extraction from Animal Tissue and Methanolysis
- 05:19Quantification of Fatty Acids by GC-FID and 13C Analysis by Isotopologue Profiling
- 08:29Results: Fatty Acid 13C Isotopologue Profiling in Collembola
- 10:32Conclusion
The fatty acid trophic marker approach, i.e., the assimilation of fatty acids as entire molecule and transfer into consumer tissue with no or minor modification, is hampered by knowledge gaps in fatty acid metabolism of small soil invertebrates. Isotopologue profiling is provided as a valuable tool to disentangle trophic interactions.
