The protocol described herein is a method for measuring endoreduplication within tubers of potato (Solanum tuberosum). It includes plasmolysis and protoplast extraction steps to decrease the noise and debris in downstream flow cytometric analysis.
Laimbeer, F. P. E., Makris, M., Veilleux, R. E. Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts. J. Vis. Exp. (133), e57134, doi:10.3791/57134 (2018).