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Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts
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JoVE Journal Biology
Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts

Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts

DOI:
10.3791/57134-v

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08:54 min

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March 09, 2018

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, ,
1Department of Horticulture,Virginia Tech, 2Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine,Virginia Tech

Chapters

  • 00:05Title
  • 00:36Day 1: Plasmolyze Tuber Tissue
  • 01:40Days 2 and 3: Generate Potato Protoplasts and Harvest and Wash Protoplasts
  • 03:42Day 3: Prepare Samples for Flow Cytometry
  • 05:07Day 3: Flow Cytometry of Potato Tuber Nuclei
  • 06:31Results: Endoreduplication of Isolated Tuber Protoplasts
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol described herein is a method for measuring endoreduplication within tubers of potato (Solanum tuberosum). It includes plasmolysis and protoplast extraction steps to decrease the noise and debris in downstream flow cytometric analysis.

Tags

Keywords: EndoreduplicationFlow CytometryPotato TubersProtoplastsCell MorphologyDevelopmental StagePlasmolysisEnzyme SolutionCellulaseMacerozymeHemicellulaseMES Buffer

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