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Journal / Biochemistry / Å avgjøre hvis DNA farget med en Cyanine Dye kan…
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Biochemistry

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JoVE Journal Biochemistry
Å avgjøre hvis DNA farget med en Cyanine Dye kan bli fordøyd med begrensning enzymer
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Å avgjøre hvis DNA farget med en Cyanine Dye kan bli fordøyd med begrensning enzymer

Article DOI: 10.3791/57141-v • 06:58 min • February 2nd, 2018
February 2nd, 2018 •
1, 1, 1, 1, 1, 1
1Department of Chemistry, University of Nebraska - Kearney

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Farging DNA molekyler for fluorescens mikroskopi kan en forsker for å vise dem i et eksperiment. Metoden som presenteres her, er DNA molekyler pre beiset med fluorescerende fargestoffer og fordøyd med metylering og ikke-metylering følsom begrensning enzymer.

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Biokjemi problemet 132 Gel geleelektroforese TOTO-1 YOYO-1 POPO-1 BOBO-1 TOTO-3 YOYO-3 POPO-3 BOBO-3 begrensning enzym fordøyelse
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