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Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

DOI:
10.3791/57148-v

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11:12 min

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August 01, 2018

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, , , ,
1Department of Pathology, Faculty of Medicine & Surgery,University of Malta, 2Centre for Molecular Medicine and Biobanking,University of Malta, 3Department of Physiology & Biochemistry, Faculty of Medicine and Surgery,University of Malta

Chapters

  • 00:04Title
  • 00:44Laser Microdissection and Tissue Lysis
  • 03:11Hybridization Magnetic Bead-based Assay
  • 06:23Reading and Analyzing the Hybridization-assay Result
  • 08:41Results: Multiplex RNA-based Expression Assay
  • 09:29Conclusion
  • 11:00Acknowledgements

Summary

Automatic Translation

Automatic Translation

Nucleic acid degradation in archival tissue, tumor heterogeneity, and a lack of fresh frozen tissue specimens can negatively impact cancer diagnostic services in pathology laboratories worldwide. This manuscript describes the optimization of a panel of biomarkers using a multiplex magnetic bead assay to classify breast cancers.

Tags

Keywords: RNA-based Expression AssayBreast CancerArchival MaterialLaser MicrodissectionFormalin-fixed Paraffin-embeddedBiomarker ValidationHybridization-based AssayMultiplexSensitivityHeterogeneous Samples

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