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JoVE Journal Bioengineering
Isolering af mesenkymale stamceller fra menneskelige alveolær Periosteum og effekter af D-Vitamin på Osteogenic aktivitet af Periosteum-afledte celler
Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
Journal (Bioengineering)
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Isolering af mesenkymale stamceller fra menneskelige alveolær Periosteum og effekter af D-Vitamin på Osteogenic aktivitet af Periosteum-afledte celler

Article DOI: 10.3791/57166-v • 06:47 min • May 4th, 2018
May 4th, 2018 •
1,2, 3, 1,4,5,6, 1,7,8
1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University

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Vi præsenterer en protokol for at undersøge mRNA udtryk biomarkører periosteum-afledte celler (fremdaterede) foranlediget af vitamin C (C-vitamin) og 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. Derudover vurderer vi fremdaterede evne til at differentiere i osteocytes, chondrocytter og adipocytter.

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Bioteknologi spørgsmålet 135 alveolær Periosteum mesenkymale stamceller 1,25-(OH)2D3 Vitamin D3 Calcitriol periost
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