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Isolamento di cellule staminali mesenchimali da periostio alveolare umano e gli effetti della vitamina D su attività osteogenica delle cellule derivate dal periostio
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1Chang Gung University, 2Department of Periodontics,Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center,Chang Gung Memorial Hospital, 5Kidney Research Center,Chang Gung Memorial Hospital, 6Center for Tissue Engineering,Chang Gung Memorial Hospital, 7Department of Periodontics,Chang Gung Memorial Hospital, 8College of Oral Medicine,Taipei Medical University
Chapters
- 00:05Title
- 01:10Tissue Preparations
- 03:15Cell Attachment and Viability with Osteogenic Differentiation
- 04:28Results: Osteogenic Differentiation of PDCs and Analysis of 1,25-dihydroxy Vitamin D (Calcitriol) and Vitamin C Treatment of PDCs using RT-PCR
- 06:11Conclusion
Vi presentiamo un protocollo per studiare i biomarcatori di espressione di mRNA di cellule derivanti dal periostio (PDC) indotte da vitamina C (vitamina C) e 1,25-diidrossi-vitamina D [1,252D-3]. Inoltre, valutiamo la capacità di differenziarsi in adipociti, condrociti e osteociti PDC.
