Journal
/
/
En omfattende Procedure at evaluere In Vitro ydeevne af den formodede Hemangioblastoma Neovascularization ved hjælp af den sfæroide, spiring Assay
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay
DOI:

08:26 min

April 12, 2018

, , , , ,

Chapters

  • 00:04Title
  • 01:46Cell Culture and Plasmid Construct
  • 03:00Lentivirus Package and Infection
  • 04:19Generation of Modified Endothelial Cell Spheroids
  • 05:04In Vitro Angiogenesis Assay
  • 06:26Results: Use of Spheroid Sprouting Assay to Study Modified Endothelial Cell Vessel Formation
  • 07:30Conclusion

Summary

Automatic Translation

Dette paper præsenterer en omfattende procedure for at evaluere in vitro- om klassiske tumor angiogenese findes i hemangioblastomas (HBs) og dens rolle i HBs. Resultaterne fremhæve kompleksiteten af HB-neovascularization og foreslå, at denne fælles form for angiogenese er kun en supplerende mekanisme i HB-neovascularization.

Related Videos

Read Article